. Author manuscript; available in PMC: 2019 Feb 4.

Published in final edited form as: Vet Pathol. 2017 Mar 27;54(4):640–648. doi: 10.1177/0300985817698207

Table 1.

Comparison of two DNA-extraction kits, and effect of adding lyticase, with respect to DNA Quality, PCR Efficiency, and Sequence Quality.

	Kit Experiment		lyticase Experiment
Measures	Qiagen	MoBio	MoBio +lyticase	MoBio -lyticase
Total number of blocks (n)	24	24	51	51
IRBP PCR Positives (n)	24	24	49	49
ITS-2 PCR Positives (n)	17	19	32	44
ITS-2 PCR Faint Bands (n)	3	1	11	2
ITS-2 Sequence Identification (n)	17	17	35	41
DNA Quality	100%	100%	94.2%	96.1%
PCR Efficiency	83.3%	83.3%	87.8%	93.9%
Sequence Identification	70.8%	70.8%	67.3%	80.4%
Correlation with histology	70.8%	70.8%	57.7%	68.6%

DNA Quality=#IRBP Positive/Total number; PCR Efficiency=(ITS-2 Positive+ITS-2 Faint)/IRBP Positive; Sequence Identification=Sequence Identification/Total number.

Qiagen: Qiagen QIAamp® DNA FFPE Tissue Kit; MoBio: MoBio BiOstic® FFPE Tissue DNA Isolation Kit

lyticase experiment: lyticase was added or not added, prior to DNA extraction using the MoBio kit.