Table 5.
Description of Acidovorax carolinensis sp. nov. according to Digital Protologue TA00473 assigned by the the www.imedea.uib.es/dprotologue website.
| TAXONUMBER | TA00473 |
| SPECIES NAME | Acidovorax carolinensis |
| GENUS NAME | Acidovorax |
| SPECIFIC EPITHET | carolinensis |
| SPECIES STATUS | sp. nov. |
| SPECIES ETYMOLOGY | ca.ro.li.nen’sis. N.L. masc. adj. carolinensis of or belonging to Carolina, refering to North Carolina, USA, where each of the strains were first isolated |
| SUBMITTER | David Singleton |
| E-MAIL OF THE SUBMITTER | drsingle@email.unc.edu |
| HAS THE TAXON BEEN SUBJECTED TO EMENDATION? | NO |
| DESIGNATION OF THE TYPE STRAIN | NA3 |
| STRAIN COLLECTION NUMBERS | =LMG 30136, =DSM 105008 |
| 16S rRNA GENE ACCESSION NUMBER | EU910093.1 |
| GENOME ACCESSION NUMBER [RefSeq] | NZ_CP021361.1 |
| GENOME STATUS | Complete |
| GENOME SIZE | 4122 kbp |
| GC mol % | 64.24 |
| COUNTRY OF ORIGIN | USA |
| REGION OF ORIGIN | Charlotte, North Carolina |
| DATE OF ISOLATION UNKNOWN (< yyyy) | 2005 |
| SOURCE OF ISOLATION | Contaminated soil |
| SAMPLING DATE | 2002–01-01 |
| GEOGRAPHIC LOCATION | Charlotte, NC former manufactured gas plant site |
| TEMPERATURE OF THE SAMPLE [In Celsius degrees] | 20 |
| pH OF THE SAMPLE | 7.5 |
| NUMBER OF STRAINS IN STUDY | 4 |
| SOURCE OF ISOLATION OF NON- TYPE STRAINS | Contaminated soil |
| GROWTH MEDIUM, INCUBATION CONDITIONS [Temperature, pH, and further information] USED FOR STANDARD CULTIVATION | Nutrient agar/R2A, 30–32.5°C, pH 7–7.5 |
| CONDITIONS OF PRESERVATION | 15% glycerol; −80°C |
| GRAM STAIN | Negative |
| CELL SHAPE | Rod |
| CELL SIZE (length or diameter) | 1.2 |
| MOTILITY | Motile |
| IF MOTILE | Flagellar |
| IF FLAGELLATED | Single, polar |
| SPORULATION (resting cells) | None |
| COLONY MORPHOLOGY | Cream color to pale yellow, circular, 1.0–1.5mm diameter. |
| TEMPERATURE RANGE | 4–40 |
| LOWEST TEMPERATURE FOR GROWTH | 4, nutrient agar plates |
| HIGHEST TEMPERATURE FOR GROWTH | 40, nutrient agar plates |
| TEMPERATURE OPTIMUM | 30–32.5 |
| LOWEST pH FOR GROWTH | 5.5, nutrient broth |
| HIGHEST pH FOR GROWTH | 8.0, nutrient broth |
| pH OPTIMUM | 7.0–7.5 |
| pH CATEGORY | Neutrophile |
| LOWEST NaCl CONCENTRATION FOR GROWTH | 0, nutrient broth |
| HIGHEST NaCI CONCENTRATION FOR GROWTH | 1%, nutrient broth |
| SALINITY CATEGORY | Nonhalophile (NaCl inhibitory at <1 % NaCl) |
| RELATIONSHIP TO O2 | Aerobe |
| O2 CONDITIONS FOR STRAIN TESTING | Aerobiosis; anaerobiosis |
| CARBON SOURCE USED [class of compounds] | Organic acids, aromatic compounds |
| CARBON SOURCE USED [specific compounds] | Phenanthrene |
| CARBON SOURCE NOT USED [specific compounds] | Acenaphthene, anthracene, benzene, ethylbenzene, fluoranthene, fluorene, naphthalene, phthalate, pyrene, salicylate, toluene, xylene |
| Positive tests with BIOLOG | Tween 40, Tween 80, Methyl pyruvate, succinic acid mono-methyl ester, β-hydroxybutyric acid, α-ketobutyric acid, α-ketoglutaric acid, α-ketovaleric acid, DL-lactic acid, succinamic acid, glycerol |
| Negative tests with BIOLOG | α-Cyclodextrin, Dextrin, Glycogen, N-Acetyl-D-galactosamine, N-Acetyl-D-glucosamine, Adonitol, L-Arabinose, D-Arabitol, D-Cellobiose, i-Erythritol, D-Fructose, L-Fucose, D- Galactose, Gentiobiose, D-Glucose, m-Inositol, α-D-Lactose, Lactulose, Maltose, D-Mannitol, D-Mannose, D-Melibiose, β-Methyl-D-Glucoside, D-Psicose, D-Raffinose, L-Rhamnose, D- Sorbitol, Sucrose, D-Trehalose, Turanose, Xylitol, Acetic Acid, Cis-Aconitic Acid, Citric Acid, Formic Acid, D-Galactonic Acid Lactone, D-Glucosaminic Acid, D-Glucuronic Acid, α- Hydroxy-Butyric Acid, γ-Hydroxy Butyric Acid, p-Hydroxy Phenylacetic Acid, Itaconic Acid, α-Keto Valeric Acid, Malonic Acid, Propionic Acid, Quinic Acid, D-Saccharic Acid, Sebacic Acid, Bromo-Succinic Acid, Glucuronamide, L-Alaninamide, D-Alanine, L-Alanine, L- Alanylglycine, L-Asparagine, L-Aspartic Acid, L-Glutamic Acid, Glycyl-L-Aspartic Acid, Glycyl-L-Glutamic Acid, L-Histidine, Hydroxy-L-Proline, L-Leucine, L-Ornithine, L- Phenylalanine, L-Pyroglutamic Acid, D-Serine, L-Serine, L-Threonine, DL-Carnitine, γ-Amino Butyric Acid, Urocanic Acid, Inosine, Uridine, Thymidine, Phenyethylamine, Putrescine, 2- Aminoethanol, D,L-α-Glycerol Phosphate, Gluose-1-Phosphate, Glucose-6-Phosphate |
| Variable tests with BIOLOG | D-galacturonic acid, D-glucuronic acid, 2,3-butanediol, L-proline |
| NITROGEN SOURCE | NH4 |
| TERMINAL ELECTRON ACCEPTOR | Oxygen |
| ENERGY METABOLISM | Chemoorganotroph |
| OXIDASE | Positive |
| CATALASE | Negative |
| POSITIVE TESTS | Lipase, nitrate reduction |
| NEGATIVE TESTS | Gelatin hydrolysis, cellulase, DNase, skim milk protease, starch hydrolysis, urease |
| QUINONE TYPE | R8 |
| MAJOR FATTY ACIDS | C16:1ω7c/C16:1ω6c, C16:0, C18:1ω7c |
| PHOSPOLIPID PATTERN OR DIAGNOSTIC PHOSPOLIPID | Phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol |
| BIOSAFETY LEVEL | 1 |
| HABITAT | Terrestrial biome |
| BIOTIC RELATIONSHIP | Free-living |
| KNOWN PATHOGENICITY | None |