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. 2019 Feb 4;14(2):e0211644. doi: 10.1371/journal.pone.0211644

Fig 3. Glutamate release of glioblastoma and brain metastasis cells.

Fig 3

In subconfluent cell cultures, supernatants (w/o FCS) were collected for a total of 24 hours (± PER) and glutamate levels were determined. Extracellular glutamate levels were normalized to total protein levels of the cells. Data are presented as mean ± SEM (n≥14), *p<0.05 vs. solvent control (Mann-Whitney U test). Multiple comparisons versus control groups (two-way ANOVA with Bonferroni t-test) demonstrated an overall higher glutamate level in the supernatant of glioblastoma cells than in the supernatant of metastasis cells (p<0.001). Additionally, the two-way ANOVA also revealed a significant treatment effect, i.e. PER attenuated extracellular glutamate levels across all cell cohorts (p = 0.046).