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. 2019 Feb 4;14(2):e0211647. doi: 10.1371/journal.pone.0211647

Fig 2. qPCR validation of genes related to ECM remodeling, wound healing, and ER homeostasis.

Fig 2

(A) The relative mRNA expression levels of selected genes related to ECM architecture and (B) involved in ER homeostasis and autophagy, were determined with the 2-(ΔΔCt) method normalized with the geometric mean of different reference genes. Bars represent the mean ratio of target gene expression in four patients’ fibroblasts compared to six unrelated healthy individuals. qPCR was performed in triplicate, and the results were expressed as mean ± SEM. **p<0.01, and ***p<0.001.