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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Methods Mol Biol. 2019;1929:397–415. doi: 10.1007/978-1-4939-9030-6_25

Figure 1.

Figure 1.

Representative 15% Tris-glycine SDS-PAGE gel of whole cell lysate of E. coli BL21(DE3) obtained from the overexpression of the S100A8 and S100A9 subunits of mCP (A)and hCP-Ser (B). Samples pre- and post-induction with IPTG are shown. The samples wereprepared using the B-PER reagent (Thermo Fisher Scientific, manufacturer protocol). The protein ladder (PL) is p7712S (New England Biolabs).