. (a) Open field test and elevated plus maze test of NS-V mice and their littermate WT control mice. Data were analyzed by two-tailed unpaired t test. nWT=10, nNS-V=8 male 4 months-old mice. (b) Elevated plus maze test. Data were analyzed by two-tailed unpaired t test. nWT=10, nNS-V=8 male 4 months-old mice. (c-d) NOR test and representative heat map. White spots indicate novel or old objects. Time duration spent in exploring each object was denoted by color from dark blue (less time) to light blue (more time). Data were analyzed by two-tailed unpaired t test. nWT=8, nNS-V=8 male 4 months-old mice. (e-f) Morris water maze test and representative heat map after the hidden platform was removed from the original location (indicated by white crosses). Data were analyzed by one-way repeated ANOVA. nWT=13, nNS-V=10 male 4 months-old mice. (g) Probe test in the water maze. Data were analyzed by two-tailed unpaired t test. nWT=13, nNS-V=10 male 4 months-old mice. (h) Ribosomal protein L10 (Rpl10) is expressed in the AAV vector with the flip-excision (FLEX) system. ITR, inverted terminal repeats. CAG, promoter. (i) AAV injection scheme. (j) RT-qPCR analysis of total and enriched mRNA (before and after immunoprecipitation, respectively). Hypothalami from 6 male 4 months-old mice were pooled for each sample in RT-qPCR analysis. n=4 samples. Data were analyzed by two-tailed unpaired t test. Data is expressed as mean ± S.E.M. For detailed statistics results, see Supplementary Table 1. * P ≤ 0.05 is set as significance.