Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. tuberculosis) | H37Rv | MRC-National Insititute for Medical Research | ||
| Strain, strain background (M. tuberculosis) | H37Rv (parent of KO) | doi: 10.1128/JB.05914–11. | Giffin et al., 2012 | |
| Strain, strain background (M. tuberculosis) | Alanine dehydrogenase KO | doi: 10.1128/JB.05914–11. | Giffin et al., 2012 | |
| Strain, strain background (M. tuberculosis) | Alanine dehydrogenase complement | doi: 10.1128/JB.05914–11. | Giffin et al., 2012 | |
| Software, algorithm | Prism 7 | GraphPad Software | ||
| Software, algorithm | Qualitative Navigator B.07.00 | Agilent software | ||
| Software, algorithm | Profinder B.08.00 | Agilent software | ||
| Chemical compound, drug | Middlebrook 7 H9 | Sigma-Aldrich | M0178 | |
| Chemical compound, drug | ADC supplement | Sigma-Aldrich | M0553 | |
| Chemical compound, drug | OADC supplement | Sigma-Aldrich | M0678 | |
| Chemical compound, drug | Middlebrook 7 H10 | Sigma-Aldrich | M0303 | |
| Chemical compound, drug | Tyloxopol | Sigma-Aldrich | T8761 | |
| Chemical compound, drug | Glycerol | Sigma-Aldrich | G5516 | |
| Chemical compound, drug | Sodium sulphate | Sigma-Aldrich | 239313 | |
| Chemical compound, drug | Sodium citrate | Sigma-Aldrich | 51804 | |
| Chemical compound, drug | Pyridoxine | Sigma-Aldrich | P9755 | |
| Chemical compound, drug | Biotin | Sigma-Aldrich | B4501 | |
| Chemical compound, drug | Sodium phosphate dibasic | Sigma-Aldrich | 71642 | |
| Chemical compound, drug | Potassium phosphate monobasic | Sigma-Aldrich | 60220 | |
| Chemical compound, drug | Ferric ammonium citrate | Sigma-Aldrich | F5879 | |
| Chemical compound, drug | Ferric citrate | Sigma-Aldrich | F3388 | |
| Chemical compound, drug | Magnesium sulphate | Sigma-Aldrich | M5921 | |
| Chemical compound, drug | Calcium chloride | Sigma-Aldrich | C8106 | |
| Chemical compound, drug | Zinc sulphate | Sigma-Aldrich | 1724769 | |
| Chemical compound, drug | Copper sulphate | Sigma-Aldrich | C6283 | |
| Chemical compound, drug | Malachite green | Sigma-Aldrich | M9015 | |
| Chemical compound, drug | L-glutamatic acid | Sigma-Aldrich | G1251 | |
| Chemical compound, drug | L-glutamine | Sigma-Aldrich | G3126 | |
| Chemical compound, drug | L-asparagine | Sigma-Aldrich | A4159 | |
| Chemical compound, drug | L-aspartatic acid | Sigma-Aldrich | A9256 | |
| Chemical compound, drug | Ammonium chloride | Sigma-Aldrich | A9434 | |
| emical compound, drug | Bromo-pyruvate | Sigma-Aldrich | 16490 | |
| Chemical compound, drug | Methionine sulfoximine | Sigma-Aldrich | M5379 | |
| Chemical compound, drug | L-Alanine-(15N2) | Cambridge Isotope Laboratory | NLM-454–1 | |
| Chemical compound, drug | L-Asparagine-(15N2) | Cambridge Isotope Laboratory | NLM-3286 | |
| Chemical compound, drug | L-Asparagine-(amine-15N) | Sigma-Aldrich | 489964 | |
| Chemical compound, drug | L-Asparagine-(amide-15N) | Cambridge Isotope Laboratory | NLM-120 | |
| Chemical compound, drug | L-Aspartate-(15N) | Sigma/Cambridge Isotope Laboratory | 332135/NLM-718 | |
| Chemical compound, drug | L-Glutamine-(15N2) | Cambridge Isotope Laboratory | NLM-31328 | |
| Chemical compound, drug | L-Glutamine-(amine-15N) | Sigma-Aldrich | 486809 | |
| Chemical compound, drug | L-Glutamine-(amide-15N) | Cambridge Isotope Laboratory | NLM-557 | |
| Chemical compound, drug | L-Glutamate-(15N) | Sigma/Cambridge Isotope Laboratory | 332143/NLM-135 | |
| Chemical compound, drug | Ammonium chloride -(15N) | Sigma-Aldrich | 299251 | |
| Chemical compound, drug | Acetonitrile | Fisher | A955-212 | |
| Chemical compound, drug | Methanol | Fisher | A456-212 | |
| Chemical compound, drug | Acetic acid | Fluka | 45740–1 L-F | |
| Sequence-based reagent | Rv0337c-fw | Integrated DNA Technologies | 5'-CACTCCGGTCCACTACCTGT-3' | qPCR primer |
| Sequence-based reagent | Rv0337c-rev | Integrated DNA Technologies | 5'- AGATCGACCATCTGGGTGAG-3' | qPCR primer |
| Sequence-based reagent | Rv0858c-fw | Integrated DNA Technologies | 5'- ACGGCACGTACTTCCTATGC-3' | qPCR primer |
| Sequence-based reagent | Rv0858c-rev | Integrated DNA Technologies | 5'- GTTCCACACATCGGCTTGTT-3' | qPCR primer |
| Sequence-based reagent | Rv1178-fw | Integrated DNA Technologies | 5'- ACGAGTGCTACCTGGGATTG-3' | qPCR primer |
| Sequence-based reagent | Rv1178-rev | Integrated DNA Technologies | 5'- AGTAGCTCGGCAACGATCTC-3' | qPCR primer |
| Sequence-based reagent | Rv1538c-fw | Integrated DNA Technologies | 5'- ACTGGAGGGACAATCTCGAC-3' | qPCR primer |
| Sequence-based reagent | Rv1538c-rev | Integrated DNA Technologies | 5'- GAGTGATGACCACCCCATCT-3' | qPCR primer |
| Sequence-based reagent | Rv2220-fw | Integrated DNA Technologies | 5'- GACAAGAGCGTGTTTGACGA-3' | qPCR primer |
| Sequence-based reagent | Rv2220-rev | Integrated DNA Technologies | 5'- GGGTCGTGCACAAAGAAGTT-3' | qPCR primer |
| Sequence-based reagent | Rv2476c-fw | Integrated DNA Technologies | 5'- GTACAGCCTGCTCGACATCA-3' | qPCR primer |
| Sequence-based reagent | Rv2476c-rev | Integrated DNA Technologies | 5'- AGCGCACCGTAAATATCGTC-3' | qPCR primer |
| Sequence-based reagent | Rv2780-fw | Integrated DNA Technologies | 5'- CTTACCACCTGATGCGAACC-3' | qPCR primer |
| Sequence-based reagent | Rv2780-rev | Integrated DNA Technologies | 5'- TAGGCCGATGAGTAGCGAGT-3' | qPCR primer |
| Sequence-based reagent | Rv3565-fw | Integrated DNA Technologies | 5'- TCTACGTGATGGACGTCTGG-3' | qPCR primer |
| Sequence-based reagent | Rv3565-rev | Integrated DNA Technologies | 5'- CACCGAGTATCCCAACTGGT-3' | qPCR primer |
| Sequence-based reagent |
Rv1221-fw | Integrated DNA Technologies | 5'- ACCATCACGACCTTGAGTCC-3' | qPCR primer |
| Sequence-based reagent | Rv1221-rev | Integrated DNA Technologies | 5'- AAAGGTCTCCTGGGTCAGGT-3' | qPCR primer |
| Sequence-based reagent | Rv2703-fw | Integrated DNA Technologies | 5'- CCTACGCTACGTGGTGGATT-3' | qPCR primer |
| Sequence-based reagent | Rv2703-rev | Integrated DNA Technologies | 5'- TGGATTTCCAGCACCTTCTC-3' | qPCR primer |
| Other | Spin-X centrifuge tube filter cellulose acetate 0.22 mM | Costar | 8160 | |
| Other | Mixed cellulose esters membrane, 0.22 mM | Millipore | GSWP02500 | |
| Other | Acid washed glass beads | Sigma-Aldrich | G1145 |