Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Cell line (H. sapiens)	MCF10A	ATCC	ATCC:CRL10317 RRID: CVCL_0598
Cell line  (H. sapiens)	Neuro2A	ATCC	ATCC:CCL131 RRID: CVCL_0470
Transfected construct	Synphilin-GFP	Zaarur et al., 2008	n/a	Generated by Sherman lab - published Zaarur et al., 2008, backbone pCXsbr
Transfected construct	Human Alpha Synuclein	Addgene	51437
Transfected construct	Dendra 2	Clonetech USA	PDendra2C
Transfected construct	Synphilin-Dendra2	This Paper	n/a	Generated from synphilin-gfp and dendra two plasmid above. Available from Cisse lab
Transfected construct	alpha Synuclein Dendra2	This paper	n/a	Generated from human alpha Synuclein and Dendra two plasmid above. Available from Cisse lab
Sequence- based reagent	siGENOME Non-Targeting siRNA #5	Dharmacon	D-001210–05
Sequence- based reagent	siGENOME RUVBL1 siRNA	Dharmacon	(D-008977–04)
Chemical compound, drug
Chemical compound, drug	MG132	Sigma Aldrich	M8699
Chemical compound, drug	Rapamycin	Sigma Aldrich	R8781
Chemical compound, drug	Cycloheximide	Sigma Aldrich	C7698
Chemical compound, drug	Azetidine-2-Carboxylic acid	Sigma Aldrich	A0760
Chemical compound, drug	Cholera Toxin	Sigma Aldrich	C8052
Chemical compound, drug	Human Insulin	Sigma Aldrich	I9278
Chemical compound, drug	Leboqitz L15 medium	Sigma Aldrich	11415064
Chemical compound, drug	Hydrocortisone	Sigma Aldrich	H0888
Chemical compound, drug	dbCAMP	Sigma Aldrich	D0627
Chemical compound, drug	Ver155008	Sigma Aldrich	SML0271
Chemical compound, drug	Lipofectamine RNAiMAX	Thermo Fisher	13778030
Chemical compound, drug	Xtremegene 9	Sigma Aldrich	6365779001
Software, algorithm	qSR	Andrews et al. (2018)		Software made available on repository - http://github.com/cisselab/qSR/
Software, algorithm	Lattice Light sheet processing code	Chen et al., 2014
Software, algorithm	Multiple Target tracking(MTT)	Sergé et al., 2008