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. 2019 Jan 4;110(2):761–770. doi: 10.1111/cas.13907

Figure 5.

Figure 5

TMEM180 expression is controlled by hypoxia in colorectal cancer (CRC) cells. A, Schema of the promoter in the TMEM180 gene. Ten hypoxia response element consensus sequences (HRE) were found in the promoter sequence. HRE are indicated by arrows. B, Western blotting of hypoxia‐inducible factor 1‐alpha (HIF‐1α). Difference in the HIF‐1α protein expression level in the CRC cell lines DLD‐1 and SW480 under normoxia (21% O2) and hypoxia (1% O2) is shown. Expression of β‐actin was used as a control. C, Quantitative RT‐PCR of TMEM180. Difference in the TMEM180 mRNA expression level in DLD‐1 and SW480 cells under normoxia (21% O2) and hypoxia (1% O2) is shown. **P < .01. Bar, SD. D,E, Immunostaining of TMEM180. Change in the TMEM180 expression pattern in DLD‐1 and SW480 cells under normoxia (21% O2) and hypoxia (1% O2) is shown (D). Scale bar, 10 μm. Data were quantified and are shown in (E). ***P < .001. Bar, SD