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. 2018 Nov 18;32(1):27–36. doi: 10.1293/tox.2018-0015

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©2019 The Japanese Society of Toxicologic Pathology

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/）.

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Fig. 4. — Immunohistochemistry of the spleen. a: Expression of PCNA protein by immunohistochemistry in the spleen. b: Integrated optical density (IOD sum) of PCNA protein expression in the spleen. Notes: A, B, and C showing the control group at 7, 14, and 21 days respectively; E, F, and H showing the AFB₁ group at 7, 14, and 21 days respectively; D and H showing negative control in the control group and the AFB₁ group respectively. Bars = 10 μm. Data are presented with the mean ± standard deviation (n = 10). * means p<0.05 compared with the control group, ** means p<0.01 compared with the control group.