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. Author manuscript; available in PMC: 2019 Dec 13.
Published in final edited form as: Cell. 2018 Nov 1;175(7):1780–1795.e19. doi: 10.1016/j.cell.2018.10.001

Figure 1. Activated T Cells Rely on Both Glucose and Glutamine to Sustain Cell Metabolism.

Figure 1.

(A) Metabolites extracted for mass spectrometry and presented as fold change from naive in T cells stimulated for 16 hr (S) or naive (N) conditions (one-way ANOVA).

(B) Oxygen consumption rate (OCR) assayed from naive CD4 cells from wild-type (WT) mice stimulated for 3 days on αCD3/CD28, injected with drug described (top). OCR at time point 200 min (bottom, one-way ANOVA).

(C and D) Abundance of metabolites (left, unpaired t test) and fractional labeling (right, one-way ANOVA) of stimulated CD4+ T cells in the presence of CB839 and 13C-glucose for glutaminolytic intermediates (C) and TCA intermediates (D).

Also see Figure S1.