(A) Normal MSCs and their differentiated adipocytes were immunohistochemically stained (brown) for nonspecific antibody (CONT AB), IGFBP2, adiponectin and CD36. (B) Expression (determined by GEP) of genes encoding IGFBP2, adiponectin, and CD36 in MSCs and their differentiated adipocytes. * p<0.03. (C) Individual primary adipocytes were captured on culture Transwell inserts (left panel) or with cytospin (right panel) and then immunohistochemically stained for IGFBP2. Images were obtained with an Olympus BH2 microscope equipped with a 160 x/0.17 numerical aperture objective; original magnification, 400×. Images were acquired with a SPOT2 digital camera and were processed with Adobe Photoshop version 11.