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. 2019 Jan 29;10:13. doi: 10.3389/fpls.2019.00013

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Copyright © 2019 Salaj, Klubicová, Matusova and Salaj.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Somatic embryogenesis in Pinus nigra. (A) zygotic embryo at responsive stage enclosed in megagametophyte explant, (B) extrusion of mucilaginous embryogenic tissue from micropylar end of megagametophyte, (C) bipolar early somatic embryo stained with acetocarmine, the embryonal cells (e) are tightly packed and the long vacuolised suspensor cells (s) are arranged into bundle, (D) early somatic embryo, the embryonal cells (e) are loosely connected and the suspensor cell (s) are without organization into bundle. Scale bars: (A,C) = 100 μm, (B) = 2 mm, (D) = 200 μm.