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. 2019 Jan 29;10:13. doi: 10.3389/fpls.2019.00013

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Copyright © 2019 Salaj, Klubicová, Matusova and Salaj.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Somatic embryogenesis in Abies hybrids. (A) Bipolar somatic embryos observed in cell line AN72, (B) besides bipolar structures also huge embryonal complexes are typical for hybrid Abies embryogenic tissues, (C) cotyledonary somatic embryos after 8–9 weeks of maturation, (D) regenerated somatic seedlings after germination, (E) somatic seedlings transferred to soil, (F) somatic seedlings growing in soil for 3–4 months (F, with permission from Salaj et al. Acta Biol. Cracov., Ser. Bot. 46, 159-167, 2004). Scale bars: (A) = 1000 μm, (B) = 200 μm, (C) = 2000 μm.