Table 1.
Initiation of somatic embryogenesis from immature zygotic embryos of Pinus nigra using different culture media.
| Medium | Years |
|||
|---|---|---|---|---|
| 2007 |
2008 |
|||
| IF | Number of initiated cell lines | IF | Number of initiated cell lines | |
| DCR | 5.2 (1.84)a,c,d | 5.0 | 9.6 (0.7)e | 13.0 |
| DCR-R | 10.4 (1.4)a,c | 14.0 | 10.3 (0.93)e | 14.0 |
| LV | 2.1 (1.4)c | 2.0 | 0.0e,f | 0.0 |
| LV-R | 0.0d | 0.0 | 0.0e,f | 0.0 |
| MLV | 8.3 (2.81)b | 8.0 | 7.4 (2.72)f | 10.0 |
| MLV-R | 7.3 (2.86)b | 7.0 | 8.8 (1.79)f | 12.0 |
| QP | 0.0b,c | 0.0 | 0.74 (0.73)e,f | 1.0 |
| QP-R | 0.0b,c | 0.0 | 0.0e,f | 0.0 |
Initiation frequencies (IF) are expressed as % of explants producing embryogenic tissues per total number of cultured explants. (With permission from Salaj et al., 2014: Dendrobiology71, 119–128).For each medium 96 explants were cultured in 2007 and 136 explants in 2008 (altogether 1856).Standard errors of mean are in parenthesis.aStatistically significant at (P 0.05) within the given year, b,c,dstatistically significant at p 0.01 within the given year, e,fstatistically significant at p 0.01 within the given year.Media DCR, LV, MLV, QP were with 9 μM 2,4-D and 2.2 μM BA.Media DCR-R, LV-R, MLV-R, QP-R were with 2.25 μM 2,4-D and 2.2 μM BA.