Figure 2.

Cigarette smoke induces lung Th17 cell and neutrophil responses. Mice were exposed to air or CS for 2 months. (A) Percentages of RORγt⁺ cells among lung CD3⁺TCRβ⁺CD4⁺ T cells. (B) Cytokines in lung homogenates as determined by Luminex assay. (C) Frequencies of neutrophils (CD11b⁺Ly6G⁺ cells) in the lung of mice exposed to air or CS for 2 months. (D) Lung mRNA expression of neutrophil response genes as determined by qRT-PCR. Data is representative of two independent experiments with 6–8 animals per group (mean ± SEM). Student's t-test (A,C,D) or Mann-Whitney test (B) was used to determine significance. ^*P ≤ 0.05; ^**P < 0.01; ^***P < 0.001.