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. 2019 Feb 5;39(2):BSR20180480. doi: 10.1042/BSR20180480

Figure 2. Efficient knockdown of Rab7a leads to suppressed cell viability and growth of MDA-MB-231 cells.

Figure 2

(A) mRNA expression of Rab7a in MDA-MB-231 cells transfected without shRNA lentivirus (Ctrl), shNC lentivirus (NC), or shRab7a lentivirus (KD1, 2, 3, and 4). **P<0.01; n=3. (B) Green fluorescence images of MDA-MB-231 cells transfected without shRNA lentivirus (Ctrl), shNC lentivirus (NC), or shRab7a lentivirus (KD2). (C) Western blots of Rab7a in cells as shown in (B). (D) Cell viability of Ctrl, shNC, and shRab7a (KD2) MDA-MB-231 cells was determined by MTT assay from day 1 to 5. **P<0.01; n=5. (E) Colony formation of indicated cells. (F) Quantitative results of colony formation in (E). **P<0.01; n=3. Abbreviation: NS, no significance.