FIGURE 1.

The oxidative effects of different concentrations of H₂O₂ on SH-SY5Y cells. (A) Cell viability was changed with the increased concentration of H₂O₂ treatment. Cell viability of the 0 μM H₂O₂ group was set to 100%. Data were presented as mean ± SEM of 6 independent experiments. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 compared with 0 μM H₂O₂ group. (B) Fluorometric assay on ΔΨm in different groups (a) and statistical analysis (b). ΔΨm was decreased with the increased concentration of H₂O₂. Fluorescence values of the 0 μM H₂O₂ was set to 0. Data were presented as mean ± SEM of 6 independent experiments. ^∗∗∗P < 0.001 compared with H₂O₂ 0 μM group. (C) Fluorometric assay on ROS levels in different groups (a) and statistical analysis (b). Fluorescence values of the 0 μM H₂O₂ group was set to 100%. Data were presented as mean ± SEM of 4 independent experiments. ^∗∗∗P < 0.001 compared with 0 μM H₂O₂ group.