FIGURE 4.

FBXL5 was involved in the degradation of IRP2 induced by H₂O₂ treatment in SH-SY5Y cells. (A,B) FBXL5 mRNA (A) and protein (B) expression were observed in SH-SY5Y cells treated by H₂O₂. One-way ANOVA, data were presented as mean ± SEM of 3 independent experiments. ^∗P < 0.05 and ^∗∗P < 0.01 compared with 0 μM H₂O₂ group. Data were presented as mean ± SEM of 3 independent experiments. One-way ANOVA, ^∗P < 0.05 and ^∗∗P < 0.01 compared with 0 μM H₂O₂ group. T-test,^#P < 0.05 and ^##P < 0.01. (C) Pretreatment with si-FBXL5, the expression of FBXL5 and IRP2 changed after treated by H₂O₂ and si-FBXL5. Data were presented as mean ± SEM of 3 independent experiments. One-way ANOVA, ^∗P < 0.05 and ^∗∗P < 0.01 compared with H₂O₂ 0 μM group. T-test,^#P < 0.05, ^##P < 0.01, and ^###P < 0.001.