Figure 9.

Systemic pro- and anti-inflammatory mediator responses following peroral MDR P. aeruginosa challenge of mice with a human gut microbiota suffering from subacute ileitis. At day 5 following subacute ileitis induction, mice with a human gut microbiota were either perorally challenged with MDR P. aeruginosa (+Psae, dissolved in PBS) or with PBS alone (Mock). Uninfected mice with a human gut microbiota but without ileitis served as control animals (Naive). 96 h following Psae challenge, (A) IFN-γ, (B) TNF, (C) IL-6, (D) MCP-1, and (E) IL-10 concentrations were determined in serum samples. Box plots represent the 75th and 25th percentiles of the median (black bar inside the boxes). The total range and significance levels determined by one-way ANOVA test followed by Tukey post-correction test for multiple comparisons are shown. The total number of analyzed animals are given in parentheses. Data were pooled from four independent experiments.