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. 2019 Jan 29;11:9. doi: 10.3389/fnagi.2019.00009

Figure 2.

Figure 2

Chaperone knockdown reduces lysosomal pool and initiates HLH-30 translocation to the nucleus. (A,B) Lysosomal pool of RNAi treated day 4 old animals. Quantification and normalization of LysoTracker-Red fluorescence (B). The error bars represent the SD of a minimum of 10 animals each. Scale bar in (A): 200 μm. (C,D) RAB-7::GFP nematodes were RNAi treated for 4 days. Upper panel shows intestinal cells and the lower panel a magnified region (C). GFP fluorescence was normalized to control RNAi (D). Error bars represent the SD of a minimum of three animals. (E,F) HLH-30::GFP animals were RNAi treated for 4 days. Percentage of animals with HLH-30::GFP nuclear translocation is shown (F). Red triangles point out nuclear HLH-30::GFP signals (E). Twenty-five animals per condition were analyzed. Scale bars: 200 μm (upper) and 50 μm (lower panel).