Figure 4.

Cordycepin-regulated UCP1 expression is AMPK-dependent. 3T3-L1 pre-adipocytes were differentiated into mature adipocytes in IDI medium then treated with or without cordycepin (10 μmol/L) for 24 h and subjected to analyses. (A) Oil red O staining. (B) Quantitative analysis of lipid content. (C) MtDNA copy number. (D) Citrate synthase activity. (E) Realtime PCR analysis of thermogenic gene expression. (F) Western blots for key proteins involved in thermogenesis. Data are displayed as mean±SEM, n=6. Significant differences compared with vehicle controls are indicated by ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 (assessed by Student׳s t-test). N.S.: nonsignficance. Cpn: cordycepin; Comp.C: compound C.