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. 2019 Feb 4;10:564. doi: 10.1038/s41467-019-08437-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Characterization of the interactions among TERB1, TERB2, and MAJIN. a Illustration of different fragments of TERB1 (upper panel). Bimolecular fluorescence complementation (BiFC) data with full-length TERB2 and varies TERB1 fragments (lower panel). Cells expressing TERB2-VC only (blue, as a negative control) or TERB2-VC with -VN vector (green, as a negative control) or TERB2-VC with different TERB1-VN fragments (red) were subjected to fluorescence-activated cell sorting (FACS) analysis. b Illustration of various fragments of TERB2 (upper panel). BiFC data with full-length TERB1 and TERB2 fragments (lower panel). Cells expressing TERB1-VC only (blue, as a negative control) or TERB1-VC with -VN vector (green, as a negative control) or TERB1-VC with different TERB2-VN truncations (red) were subjected to FACS analysis. c Equator images of U-2 OS cells expressing SUN1-GFP (green) together with mCherry-MAJIN (red) (up) or Flag-TERB2 (magenta) (down). SUN1-GFP was used to indicate the nuclear envelope localization. Cells were stained with 4,6-diamidino-2-phenylindole (DAPI; blue) to show the nuclear position. Scale bars, 10 μm. d Schematics of various TERB2 fragments (upper panel). Equator images of U-2 OS cells expressing mCherry-MAJIN (red) and Flag-TERB2 truncations. Cells were stained with Flag antibody (magenta) and DAPI (blue). Scale bars, 10 μm. e Gel filtration chromatography profile of the TERB2_MBM–MAJIN_NTD complex. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis image of the peak in the gel filtration chromatography profile is shown