Fig. 10.

Speedy A and SUN1 exhibit congregation in Terb2 mutant spermatocytes. a, c Immunofluorescence–fluorescence in situ hybridization (FISH) showing distribution of Speedy A (a) and SUN1 (c) in spermatocytes. Sections of 2-week-old testes of different genotypes as indicated were immunostained for SYCP3 and Speedy A (a) or SYCP3 and SUN1 (c) and subjected to telomere FISH (TelC). DNA was stained by 4,6-diamidino-2-phenylindole. White arrowheads indicate congregation of Speedy A (a) or SUN1 (c). White arrows in a indicate Speedy A signals co-localized with telomeres. Scale bars, 5 μm. b Population of nuclei that display Speedy A congregation in a. Data are from three independent experiments with different mice and error bars indicate s.d. d Population of nuclei that display SUN1 congregation in c. Data are from three independent experiments with different mice and error bars indicate s.d. For each experiment, >100 spermatocytes from each indicated genotype were counted for the frequencies of nuclei showing congregation of Speedy A (b) or SUN1 (d). Source data are provided as a Source Data file