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. 2019 Feb 4;10:564. doi: 10.1038/s41467-019-08437-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Mutational analyses of the TERB1–TERB2 and TERB2–MAJIN interactions. a, c Detailed interactions around Tyr56^TERB2 in the TERB1_T2BM–TERB2_NTD complex (a) and around Phe192^TERB2 in the TERB2_MBM–MAJIN_NTD complex (c). b, d Co-immunoprecipitation (Co-IP) of human TERB1 (b) or MAJIN (d) with wild-type (WT) or mutant Flag-tagged human TERB2 in 293T cells. The levels of each protein in the input and IP samples were analyzed by immunoblotting with the indicated antibodies. Input contains 5% of the input whole-cell lysate used for IPs. e Multiple sequence alignment of the N-terminal (top) and C-terminal (bottom) regions of TERB2 required for its association with TERB1 and MAJIN, respectively. Sequence positions are shown based on the human TERB2. The residues of Tyr56 (Try56 in mouse) and Phe192 (Phe188 in mouse) are indicated by blue arrowheads. f, g Co-IP of mouse TERB1 (f) or mouse MAJIN (g) with WT or mutant Flag-tagged mouse TERB2 in 293T cells. The levels of each protein in the input and IP samples were analyzed by immunoblotting with the indicated antibodies. Input contains 5% of the input whole-cell lysate used for IPs. Source data are provided as a Source Data file