Fig. 9.

Telomere localization of Speedy A and CDK2 is reduced in mutant nuclei. a Immunofluorescence–fluorescence in situ hybridization (IF-FISH) images of chromosome spreads from adult spermatocytes of the indicated genotypes. Chromosome spreads were immunostained for SYCP3 (green) and Speedy A (red) and subjected to telomere FISH (TelC-FISH) (magenta). DNA was stained by 4,6-diamidino-2-phenylindole (DAPI; blue). Zyg Zygotene, Pac Pachytene, En showing enhanced signals (red) of Speedy A with a lower cutoff. White arrowheads indicate sex chromosomes. Scale bars, 10 μm. b Quantification of the relative intensity of Speedy A foci at telomeres in wild-type (WT) or Terb2-mutant spermatocytes in a. c IF-FISH images of chromosome spreads from adult spermatocytes of the indicated genotypes. Chromosome spreads were immunostained for SYCP3 (green) and CDK2 (red) and subjected to telomere FISH (TelC-FISH) (magenta). DNA was stained by DAPI (blue). Zyg Zygotene, Pac Pachytene, En showing enhanced signals (red) of CDK2 with a lower cutoff. White arrowheads indicate sex chromosomes. Scale bars, 10 μm. d Quantification of the relative intensity of CDK2 foci at telomeres in WT or Terb2-mutant spermatocytes in c. Source data are provided as a Source Data file