Fig. 2.

Heterologous protein expression and promoter evaluation in SR7. a Schematic of the promoters tested in SR7. pXyl is a xylose-inducible promoter and contains the xylR gene and xylA promoter from B. megaterium strain DSM 319. pHysp contains the IPTG-inducible hyperspank promoter and lacI, both from plasmid pDR111. The p43 promoter is taken from B. subtilis strain KS438 and is growth-associated. Expected inducers are indicated in parentheses. b Bulk fluorescence measurements of SR7 populations containing gfp-encoding plasmids, with (circles, solid lines) or without (diamonds, dashed lines) inducers. Error bars represent the standard deviation of biological triplicate samples. c Fluorescence populations of SR7 cells expressing gfp at 4 h post induction measured by FACS (Alexa Fluor 488). A GFP positive gate was established using the autofluorescence of SR7 cells containing an empty plasmid control. d Phase-contrast and fluorescence microscopy of SR7 pXyl gfp cells with and without xylose induction. Images were taken 4 h post induction. Source data are provided as a Source Data file