Fig. 3.

β-Galactosidase activity for SR7 cultures grown under 0.1 MPa CO₂ and 10 MPa scCO₂ at 37 °C. Specific β-galactosidase activity measured in lysates of SR7 cultures that contain an empty pXyl plasmid or pXyl lacZ. Activity was normalized to total protein concentration in the lysate to control for growth differences and differential cell lysis. Errors represent standard deviation of biological replicate cultures (n > 4). For cultures grown under 0.1 MPa CO₂, overnight anaerobic cultures grown from spores were subcultured into CO₂-evacuated vials to a uniform optical density. Induction with xylose occurred 2 h after subculture, and the time point for LacZ activity measurement was taken 4 h post induction. For cultures grown under 10 MPa scCO₂, SR7 pXyl lacZ spores were loaded into stainless steel culture vessels with and without xylose inducer. Cultures were grown for 21 days and microscopy was used to determine which samples showed at least a tenfold increase in cell number prior to measuring LacZ activity. Source data are provided as a Source Data file