Fig. 6. Essential molecules promote angiogenesis mediated by β-arrestin-2-dependent pathway with or without ischemia.
a Protein array related to angiogenesis was examined using β-arrestin-2 knockdown cells (miR βarrestin-2) under ischemic condition (1% O2 3 h) compared with control cells (miR Cont). After 18 h from OGD treatment, representative array membranes showed. Six of total 31 angiogenesis-related proteins with significant change were marked with square boxes and labeled. b Densitometric analysis revealed low level of Cyr61/CCN, AMDMTS1, Angiogenin, Endothelin-1, PDGF-AA and PDGF-AB/BB in β-arrestin-2 knockdown cells (miR β arrestin-2) compared with respective controls (miR Cont). c Results of quantitative RT-PCR of PDGF-α and PDGF-β at β-arrestin-2 knockdown cells (miR β arrestin-2) were showed compare with control HUVECs (miR Cont). Results are means ± SEM (n = 4). Data were analyzed compared with multiple groups by Kruskal–Wallis tests. *p < 0.05