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. 2019 Jan 18;10(2):52. doi: 10.1038/s41419-018-1288-0

Fig. 5. CBX8 promotes stem cell-like behavior of HCC cells.

Fig. 5

a Holoclone assays in HepG2 cells transfected with pBabe or pBabe-CBX8, and SMMC-7721 cells transfected with pSuper or pSuper-shCBX8 were used in three experiments (Exp. I, 100 cells/well scored on d 9; Exp. II, 100 cells/well scored on d 13; Exp. III, 500 cells/well scored on d 7). b Clonogenic assay (Exp. I, 1250 cells/well scored on d 5; Exp. II, 25,000 cells/well scored on d 5) in HepG2 cells transfected with pBabe or pBabe-CBX8 and SMMC-7721 cells transfected with pSuper or pSuper-shCBX8. Two experiments were performed (Exp. I, 1250 cells/well scored on d 5; Exp. II, 25,000 cells/well scored on d 5). c SP population in CBX8 overexpression and silenced HCC cells was determined by Hoechst 33342 efflux assay. d The expression of CBX8 mRNA in sorted primary EPCAM− and EPCAM+ liver cancer cells was determined by qRT-PCR (left). The expression of CBX8 mRNA of sorted primary CD133− and CD133+ liver cancer cells was determined by qRT-PCR (right). e Levels of CBX8 mRNA in magnetically sorted EPCAM+ and EPCAM− were measured by qRT-PCR in HepG2 and SMMC-7721 cells (left). Levels of CBX8 mRNA in magnetically sorted CD133+ and CD133− were measured by qRT-PCR in HepG2 and SMMC-7721 cells (right). **p < 0.01. CBX8 Chromobox homolog 8, HCC hepatocellular carcinoma, qRT-PCR quantitative reverse transcription-PCR, mRNA messenger RNA