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. 2019 Jan 18;10(2):52. doi: 10.1038/s41419-018-1288-0

Fig. 6. CBX8 regulates BMP4 expression through H3K27 trimethylation.

Fig. 6

a Supervised hierarchical clustering of the differentially expressed genes after CBX8 silencing in SMMC-7721 cells. b BMP4 mRNA expression was measured by qRT-PCR and BMP4 protein expression was measured by western blotting in HCC cells with CBX8 overexpression or knockdown. c H3K4me3, H3K9me3, and H3k27me3 was measured by western blotting. d Presentation of five regions relative to the BMP4 transcriptional start site used as primers to test histone occupied abundance. e BMP4 is one of the genes that bind on CBX8 protein by ChIP-seq. f The relative methylation of the BMP4 promoter in HCC tissues. Representative results are shown between adjacent liver tissues and HCC tissues, and the comparison of the relative methylation of the BMP4 promoter between HCC tissues with or without metastasis. **p < 0.01. CBX8 Chromobox homolog 8, BMP4 bone morphogenetic protein 4, mRNA messenger RNA, qRT-PCR quantitative reverse transcription-PCR, ChIP-seq chromatin immunoprecipitation-sequencing