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. 2019 Feb 4;9:1122. doi: 10.1038/s41598-018-37692-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical detection of filamin A (FLNA) in somatotropinomas. Representative images of FLNA immunohistochemical scores in somatotropinomas at 400X magnification: (A) Score-0: no membrane or cytoplasmic immunoreactivity; (B) Score 1: low membrane and cytoplasmic immunoreactivity; score 2: moderate membrane and cytoplasmic immunoreactivity; Score 3: high membrane and cytoplasmic immunoreactivity. Scale bar: 100 µm. In addition, negative (E) and positive (F) controls of the IHC validation are represented using a FFPE-uterus sample. (G) Correlation between FLNA IHC-staining and mRNA levels).