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. 2019 Feb 4;9:1112. doi: 10.1038/s41598-019-38617-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The experimental design in this study. Three different experimental designs were used in this study. In the first one, gDNA of P. afer and X. nigri were used for hybridization against chromosomal background of N. notopterus. In the second set of experiments, gDNA of X. nigri was used for hybridization against chromosomal background of P. afer and in the third set of experiments, gDNA probes of all Notopteridae species were used for hybridization against chromosomal background of C. lopis.