Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 4;9:1359. doi: 10.1038/s41598-018-37656-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Design of an assay to quantify the activity of an enzyme that is a key regulator of bile acid homeostasis. (A) Bile salt hydrolase activity of the gut microbiota modulates the properties of bile salts, which are produced by the liver. BSH facilitates hydrolysis of the amide bond between the bile acid and glycine/taurine. (B) Chemical structures for cholic acid–AMCA probe (CA-AMCA), and proposed strategy for continuous monitoring of BSH activity. The probe was created by a one-step chemical synthesis in which a purified bile acid was coupled to 7-amino-4-methyl-3-coumarinylacetic acid, and subsequently purified by reverse-phase HPLC.