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. 2019 Feb 4;9:1359. doi: 10.1038/s41598-018-37656-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biochemical characterization of a synthetic BSH substrate (CA-AMCA). (A) Substrate affinity was assessed through generation of a Michaelis-Menten curve by treating a buffered solution of purified BSH with varying concentrations of CA-AMCA and plotting the turnover rates against substrate concentration, and (B) K_i for caffeic acid phenylethyl ester (CAPE), which is a previously reported small molecule inhibitor of BSH.