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. 2019 Feb 4;9:1359. doi: 10.1038/s41598-018-37656-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The CA-AMCA assay is capable of detecting endogenously expressed BSH both in cell lysates and in vivo for L. plantarum, which is a BSH-expressing habitant of human gut. Standard deviation for n = 3 replicates are shown superimposed on line that connects the measured averages. (A) CA-AMCA (150 μM) was added to buffered solutions containing varying amounts of L. plantarum lysate. (B) CA-AMCA (150 μM) was added to buffered solutions containing varying amounts of intact L. plantarum cells. Rates of CA-AMCA turnover are dependent on cell density.