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CA-AMCA robustly reports on BSH activity in the context of human fecal microbiome lysates. CA-AMCA (150 µM) was added to a prepared sample of buffered human gut microbiome lysate (500 µg/ml protein) with purified BSH (20 µg/ml) added, and substrate turnover was monitored using a fluorescence plate reader. Standard deviation for n = 3 replicates are shown superimposed on line that connects the measured averages.
