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. 2019 Feb 4;9:1161. doi: 10.1038/s41598-018-37894-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the PU.1 binding region in the mouse Ccl22 promoter. ChIP assay was performed using either goat IgG (gIgG) or anti-PU.1 Ab (PU.1) in BMDCs (a), BMDMs (b), splenic DCs (c), and peritoneal macrophages (d). The amounts of immunoprecipitated chromatin were determined by quantitative PCR amplifying the indicated region of the Ccl22 promoter. Data are expressed as the percentage of the input for each ChIP assay. Results are shown as means + S.D.s (n > 3). Similar results were obtained in more than three independent experiments. *p < 0.05.