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. 2019 Feb 4;9:1282. doi: 10.1038/s41598-018-38004-5

Figure 1.

Figure 1

Effect of cisplatin and metformin on human NSCLC cell proliferation. (A,B) A549 and H460 human NSCLC cells were exposed to increasing concentrations (0–5 µM) of cisplatin (CP) and counted at 24, 48 and 72 h after cisplatin treatment. Proliferation results of 3–4 independent experiments are shown. Statistically significant differences compared with corresponding control cells (not treated with cisplatin) are shown (*P < 0.05). (C,D) A549 and H460 human NSCLC cells were treated with increasing concentrations of metformin (2 µM–8 mM) and cells were counted 48 h later. Proliferation results of 3–4 independent experiments are shown. Statistically significant differences compared with corresponding control cells (not treated with metformin) and 48 h metformin treatment groups are shown (*P < 0.05). (E) A549 and H460 cells were pre-treated with 1 and 2 mM metformin (MET), respectively, for 24 h followed by cisplatin (CP, 1 µM) for 4 h and subjected to 2 Gy of radiation. Cells were counted 24 h after radiation. Metformin was present in the medium after radiation. Results of 3–4 independent experiments are shown. Statistically significant differences of drug treated cells from untreated control cells (−) are shown: *P < 0.05 vs. untreated respective controls.