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. 2019 Feb 4;9:1282. doi: 10.1038/s41598-018-38004-5

Figure 6.

Figure 6

Effect of metformin on ERCC1 expression in NSCLC cells. A549 and H460 cells were treated with 1 mM and 2 mM of metformin, respectively, for 0–48 h, lysed and probed with anti-ERCC1 and anti-GAPDH antibodies. Representative immunoblots (top) and the corresponding densitometric quantification (bottom) from 3–4 independent experiments are shown. *P < 0.05 compared to untreated cells (Ctr) (A,C). A549 and H460 cells were treated with metformin (0.1–2 mM) for 48 h, lysed and probed with anti-ERCC1 and anti-GAPDH antibodies. Representative immunoblots (top) and the corresponding densitometric quantification (bottom) from 3–4 independent experiments are shown. *P < 0.05 compared to untreated cells (Ctr) (B,D). A549 and H460 cells were pre-treated with 1 mM and 2 mM of metformin (MET), respectively, for 24 h with or without CP (1 µM) for further 4 h, followed by exposure to 2 Gy of radiation. Cells were lysed 24 h after radiation and probed with the indicated antibodies. Representative immunoblots (top) and the corresponding densitometric quantification (bottom) from 3–4 independent experiments are shown. *P < 0.05 compared to only irradiated cells (Ctr) (E,F).