Figure 3.

Alka/ene hydrocarbon content of the S7002 strains expressing Ols and Ado/Aar pathways and WT references. Hydrocarbon content was normalized as percent of dry cell weight (% DCW). (A) S7942 at 38 °C. (B) S6803 at 30 °C. (C) 7002::control at 38 °C. (D) 7002::6803alk at 38 °C. (E) 7002::trc7942alk at 38 °C. (F) 7002::control at 27 °C. (G) 7002::6803alk at 27 °C. (H) 7002::trc7942alk at 27 °C. (I) 7002::control at 25 °C. (J) 7002::6803alk at 25 °C. (K) 7002::trc7942alk at 25 °C. A to H were cultured under 300 μmol photons s⁻¹ m⁻² and bubbled with 5% CO₂-supplemented air and I to K at 50 μmol photons s⁻¹ m⁻² in air. S6803 was cultured at 30 °C since the strains is not tolerant of 38 °C. Error bars are 1 SD based on at least three biological replicates.