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. 2019 Feb 4;9:1360. doi: 10.1038/s41598-018-38124-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alka/ene content of 7002ΔOls substituted with non-native hydrocarbon biosynthetic genes presented as % DCW. (A) ΔOls::6803alk at 38 °C. (B) ΔOls::7942alk at 38 °C. (C) ΔOls::UndA at 38 °C. (D) ΔOls::FAP at 38 °C. (E) ΔOls::6803alk at 27 °C. (F) ΔOls::7942alk at 27 °C. (G) ΔOls::UndA at 27 °C. (H) ΔOls::FAP at 27 °C. Strains were cultured as in Fig. 3. Error bars are 1 SD based on at least three biological replicates.