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. 2019 Feb 4;9:1393. doi: 10.1038/s41598-018-37476-9

Figure 2.

Figure 2

Effects of SDCs on Aβ1–42 fibrillation. WT K562 cells and SDC transfectants were incubated with Aβ1–42 at a concentration of 5 μM for 1 h to 18 h at 37 °C. (a) After 1, 3, 6 and 18 h of incubation, the cells were treated with ThT at a concentration of 15 μM for 10 mins and fluorescence was measured. Amyloid fluorescence is expressed as fold change over background ThT fluorescence. The bars represent mean ± SEM of four independent experiments. Statistical significance vs Aβ1–42-treated WT K562 cells was assessed by analysis of variance (ANOVA). *p < 0.05 vs Aβ1–42-treated WT K562 cells; **p < 0.01 vs Aβ1–42-treated WT K562 cells. (b) Scanning electron microscope visualization of WT K562 cells and SDC transfectants treated with Aβ1–42 at a concentration of 5 μM for 1, 6 or 18 h. Representative images of three independent experiments are shown. Scale bar = 1 μm. (c) CLSM visualization of ThT labeled, intracellular Aβ1–42 fibrils in WT K562 cells and SDC transfectants. Representative images of three independent experiments are shown. Scale bar = 5 μm.