Fig. 6. miR-29c-3p regulates the expression of large tumor suppressor gene 1 (LATS1) via DNA methyltransferase 3B (DNMT3B).
a mRNA expression levels of miR-29c-3p, DNMT3B and LATS1 after transfection with pre-miR-29c-3p and miR-29c-3p inhibitor in MHCC-97H cells. b After transfection with pre-miR-29c-3p and miR-29c-3p inhibitor, DNMT3B, LATS1, p-YAPs127, and YAP expression levels were detected by western blot. c After transfection with pre-miR-29c-3p and miR-29c-3p inhibitor, DNMT3B and LATS1 expression in MHCC-97H cells was detected by immunofluorescence. d The methylation status of LATS1 after transfection with pre-miR-29c-3p in MHCC-97H cells was detected by methylation-specific PCR (MSP) and bisulfite sequence‑PCR (BSP); *p < 0.05, **p < 0.01. Black dots, methylation, white dots, unmethylated