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. 2019 Jan 18;10(2):46. doi: 10.1038/s41419-018-1295-1

Fig. 4. Regulation of the endogenous protein level of NFΚBIA by an altered miR-34a expression in CD8+ T cells.

Fig. 4

a, b Western blot analysis of the impact of altered miR-34a-levels on the endogenous NFΚBIA protein level in CD8+ cells. CD8+ T cells were transfected either with ANC or miR-34a-5p mimic (a) and with inhibitor control (IC) or anti-miR-34a-5p (b). 48 h after transfection, the endogenous protein level of NFΚBIA was analyzed by western blotting using specific antibodies against NFΚBIA. GAPDH served as loading control. c, d Quantification of endogenous NFΚBIA protein levels in CD8+ T cells with altered miR-34a expression. The NFΚBIA protein expression in miR-34a transfected CD8+ T cells (c) and in anti-miR-34a transfected CD4+ T cells (d) was quantified by densitometry using Image Lab Software. Three independent western blot experiments were quantified each. The expression of NFΚBIA was normalized to the corresponding GAPDH signals of the respective samples. One asterisks correspond to p < 0.05. Data are represented as mean ± SD