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. 2019 Feb 4;9:1281. doi: 10.1038/s41598-019-39018-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of IL13Rα2 expression on the in vitro proliferation of SK-MEL-28 melanoma cells. The SK-MEL-28 cells were transfected with the expression plasmid encoding IL13Rα2 to establish SK-IL13Rα2 to study the effect of IL13Rα2 on cell proliferation. (A) The expression of IL13Rα2 in the SK-MEL-28, SK-IL13Rα2 and A375 (IL13Rα2-positive) cells was determined by qRT-PCR. (B) 2 × 10⁴ of SK-MEL-28 and SK-IL13Rα2 cells were seeded into 6-well plates and were allowed to grow for 5 days. The cells were harvested and were counted on the indicated day. All values are shown as the ratios to the number of the seeded cells on day 0 and are mean ± SD. *p < 0.05; Student’s t-test.