Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 4;9:1281. doi: 10.1038/s41598-019-39018-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effects of IL13Rα2 on amphiregulin expression in various types of melanoma cells. (A) Total RNAs were prepared from tumour tissues derived from the SK-MEL-28 (#1, #2) and SK-IL13Rα2 (#7, #8) cells and were subjected to quantitative RT-PCR analyses for the expression of IL13Rα2 (top) and amphiregulin (bottom). (B) The A2058 and SK-MEL-28 melanoma cells were transfected with IL13Rα2 expression vector, followed by qRT-PCR analysis for the expression of IL13Rα2 (top) and amphiregulin (bottom). (C) IL13RA2 was knocked out in the A375 melanoma cells, followed by qRT-PCR analysis for the expression of IL13Rα2. (D) The A375 melanoma cells were transfected with negative control (NC) siRNAs or siRNAs for IL13Rα2 (#1 and 2), followed by qRT-PCR analysis for the expression of IL13Rα2 (top) and amphiregulin (bottom). All values are mean ± SD. *p < 0.05; Student’s t-test.