Fig. 8. PEDF suppresses components in the CASP2-dependent apoptotic pathway after ONC in vivo.

a CASP2-dependent apoptosis requires the formation of the PIDDosome (Adapted from²⁴). b PEDF treatment suppresses CASP2, RAIDD but not PIDD and downstream Bim mRNA. c, d PEDF treatment suppresses C-CASP2, RAIDD, phopho-c-Jun and downstream Bim as detected by western blot and densitometry. e, f Immunohistochemistry to show changes in Bim protein colocalised to caspase-2 in the ganglion cell layer (GCL), inner plexiform layer (IPL), occasional cells of the inner nuclear layer (INL) whilst high power views of the GCL showed that Bim and caspase-2 were colocalised to RGC. Scale bars in e and (f) = 50 µm (n = 18 retinae/treatment)