Table 1.
List of primary and secondary antibodies used for western blot (WB), immunohistochemistry (IHC) and immunocytochemistry (ICC)
| Antibody | Source | Cat No. | Dilution | |
|---|---|---|---|---|
| Primary antibodies | ||||
| WB | IHC/ICC | |||
| Goat anti-cleaved caspase-2 (p12) | Santa Cruz Biotechnology, San Diego, CA, USA | sc-623 | 1:200 | 1:200 |
| Rabbit anti-cleaved caspase-3 | Cell Signalling Technology, Danvers, MA, USA | 9664 | 1:200 | |
| Rabbit anti-cleaved caspase-6 | Cell Signalling Technology, Danvers, MA, USA | 9761 | 1:200 | |
| Rabbit anti-cleaved caspase-7 | Cell Signalling Technology, Danvers, MA, USA | 8438 | 1:200 | |
| Rabbit anti-cleaved caspase-8 | Cell Signalling Technology, Danvers, MA, USA | 9496 | 1:200 | |
| Rabbit anti-RAIDD | Abcam, Cambridge, UK | ab76465 | 1:1000 | |
| Rabbit anti-PIDD | Merck Millipore, Watford, UK | MACB189 | 1:1000 | |
| Rabbit anti-Phospho-c-Jun(Ser63) | Cell Signalling Technology, Danvers, MA, USA | 2361 | 1:750 | |
| Rabbit anti-c-Jun | Cell Signalling Technology, Danvers, MA, USA | 9165 | 1:750 | |
| Rabbit anti-Bim | Cell Signalling Technology, Danvers, MA, USA | 2933 | 1:1000 | |
| Mouse anti-βIII tubulin | Sigma, Poole, UK | T8660 | 1:500 | |
| Mouse anti-β actin | Sigma, Poole, UK | A5441 | 1:400 | |
| Secondary antibodies | ||||
| HRP-labelled anti-mouse IgG | GE Healthcare, Buckingham, UK | NA931 | 1:1000 | |
| HRP-labelled anti-rabbit IgG | GE Healthcare, Buckingham, UK | NA934 | 1:1000 | |
| HRP-labelled anti-goat IgG | Santa Cruz Biotechnology, San Diego, CA, USA | sc-2020 | 1:1000 | |
| Alexa594 anti-goat IgG | Invitrogen, Poole, UK | A-11058 | 1:400 | |
| Alexa488 anti-mouse IgG | Invitrogen, Poole, UK | A-11001 | 1:400 | |
| Alexa488 anti-rabbit IgG | Invitrogen, Poole, UK | A-21206 | 1:400 | |