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. 2019 Feb 4;9:1201. doi: 10.1038/s41598-018-37874-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of synapsin genes in the mouse striatum change after RF-EMF exposure. (A–C) Striatal total RNA was extracted from sham-exposed and RF-EMF-exposed mice and analyzed for synapsin I/II/III expression levels. The relative mRNA levels of synapsin I/II/III were normalized to the expression level of GAPDH by the 2^−ΔΔCt method. (D) Shown are the expression levels of synapsin genes by sqRT-PCR followed by electrophoresis on 1.5% agarose gel. Cropped, representative gel images are shown. The expression values in the striatum of RF-EMF-exposed mice were normalized to those of the sham-exposed mice. Each bar represents the mean ± SEM of three independent experiments. Statistical significance was evaluated using a two-tailed, unpaired Student’s t-test (*P < 0.05; **P < 0.01).